Warning: Illegal string offset 'html' in /home/hsn/public_html/forum/cache/skin_cache/cacheid_1/skin_topic.php on line 909

Warning: Cannot modify header information - headers already sent by (output started at /home/hsn/public_html/forum/cache/skin_cache/cacheid_1/skin_topic.php:909) in /home/hsn/public_html/forum/admin/sources/classes/output/formats/html/htmlOutput.php on line 114

Warning: Cannot modify header information - headers already sent by (output started at /home/hsn/public_html/forum/cache/skin_cache/cacheid_1/skin_topic.php:909) in /home/hsn/public_html/forum/admin/sources/classes/output/formats/html/htmlOutput.php on line 127

Warning: Cannot modify header information - headers already sent by (output started at /home/hsn/public_html/forum/cache/skin_cache/cacheid_1/skin_topic.php:909) in /home/hsn/public_html/forum/admin/sources/classes/output/formats/html/htmlOutput.php on line 136

Warning: Cannot modify header information - headers already sent by (output started at /home/hsn/public_html/forum/cache/skin_cache/cacheid_1/skin_topic.php:909) in /home/hsn/public_html/forum/admin/sources/classes/output/formats/html/htmlOutput.php on line 137

Warning: Cannot modify header information - headers already sent by (output started at /home/hsn/public_html/forum/cache/skin_cache/cacheid_1/skin_topic.php:909) in /home/hsn/public_html/forum/admin/sources/classes/output/formats/html/htmlOutput.php on line 141
Chromatography - HSN forum

Jump to content


Chromatography


5 replies to this topic

#1 **Bernadette**

    Good Effort

  • Members
  • PipPipPip
  • 80 posts
  • Location:Bellshill
  • Interests:I am currently in sixth year and I am doing higher music and advanced highers in maths chemistry and biology.<br />In fourth year I got six 1s in english, chemistry, biology, music, administration and modern studies. I also got 2 As in the int 2s I sat in french and maths. Last year I got five A's!! Woohoo!! I also sit piano exams so I have passed up to grade 6 practical and grade 5 theory so far. I would like to go on and study medicine.<br /><br />I like playing the piano, playing drums, dancing(tap, ballet, modern, highland, disco...), watching golf, tennis(I am not very good at playing but I love Wimbledon) reading, doing crosswords and loadsa other stuff! I am also trying to teach myself guitar which is funny!! I love music and I am going to see the Fratellis in feb and Razorlight in April!! Can't wait!! Some of my other favourite bands are snow patrol, blur, bloc party, radiohead, the kooks, oasis and of course the Beatles!!!!
  • Gender:Female

Posted 22 October 2006 - 09:36 PM

Hi I was wondering if there are any other solvents that can be used for developing amino acids after chromatography instead of ninhydrin?

#2 ginneswatson

    Site Swot

  • Members
  • PipPipPipPip
  • 143 posts
  • Location:Kelso
  • Gender:Male

Posted 25 October 2006 - 07:22 PM

QUOTE(**Bernadette** @ Oct 22 2006, 10:36 PM) View Post

Hi I was wondering if there are any other solvents that can be used for developing amino acids after chromatography instead of ninhydrin?


Hi biggrin.gif

I'm not sure but there are bound to be other options. Why do you not want to use ninhydrin?

Anyway, I'll root about and see what I can find.

Cheers

#3 ginneswatson

    Site Swot

  • Members
  • PipPipPipPip
  • 143 posts
  • Location:Kelso
  • Gender:Male

Posted 25 October 2006 - 07:45 PM

You can use 1,2-Naphthoquinone-4-sulfonic acid sodium salt , aka 3,4-Dihydro-3,4-dioxo-1-naphthalenesulfonic acid sodium salt , known to its friends as Folin's Reagent or C10H5NaO5S for short.

Sigma-Aldrich stock it http://www.sigmaaldrich.com/catalog/search...382&Brand=FLUKA but do not quote a price online (bad sign ? unsure.gif )

If it turns out to be another name for ninhydrin, I apologise but I think it is a genuine alternative.

Cheers

#4 **Bernadette**

    Good Effort

  • Members
  • PipPipPip
  • 80 posts
  • Location:Bellshill
  • Interests:I am currently in sixth year and I am doing higher music and advanced highers in maths chemistry and biology.<br />In fourth year I got six 1s in english, chemistry, biology, music, administration and modern studies. I also got 2 As in the int 2s I sat in french and maths. Last year I got five A's!! Woohoo!! I also sit piano exams so I have passed up to grade 6 practical and grade 5 theory so far. I would like to go on and study medicine.<br /><br />I like playing the piano, playing drums, dancing(tap, ballet, modern, highland, disco...), watching golf, tennis(I am not very good at playing but I love Wimbledon) reading, doing crosswords and loadsa other stuff! I am also trying to teach myself guitar which is funny!! I love music and I am going to see the Fratellis in feb and Razorlight in April!! Can't wait!! Some of my other favourite bands are snow patrol, blur, bloc party, radiohead, the kooks, oasis and of course the Beatles!!!!
  • Gender:Female

Posted 26 October 2006 - 07:23 PM

Thanks for the advice!! My teacher wasn't to keen on using it because it was carcinogenic. It has been used before in the school but always by teachers. I think he was a bit wary of a pupil using it.

#5 ross_rosco

    Showing Improvement

  • Members
  • PipPip
  • 27 posts
  • Location:Dundee
  • Interests:Music (writing, listning, playing) <br />Football - mildly interested<br />Hockey - Play for men's team
  • Gender:Male

Posted 26 October 2006 - 07:26 PM

undermining or what!?
"It's Five O'Clock Somewhere..."

#6 YIC

    Fully Fledged Genius

  • Members
  • PipPipPipPipPipPipPip
  • 1,976 posts
  • Location:UK
  • Interests:My interests
  • Gender:Male

Posted 13 November 2006 - 04:02 AM

if you want to develop amino acids without ninhydrin, try using iodine crystals, but do it in a closed jar. This might not be as good as ninhydrine, but it should be fine.





1 user(s) are reading this topic

0 members, 1 guests, 0 anonymous users