Hi I was wondering if there are any other solvents that can be used for developing amino acids after chromatography instead of ninhydrin?


Chromatography
Started by **Bernadette**, Oct 22 2006 09:36 PM
5 replies to this topic
#1
Posted 22 October 2006 - 09:36 PM
#2
Posted 25 October 2006 - 07:22 PM
Hi I was wondering if there are any other solvents that can be used for developing amino acids after chromatography instead of ninhydrin?
Hi

I'm not sure but there are bound to be other options. Why do you not want to use ninhydrin?
Anyway, I'll root about and see what I can find.
Cheers
#3
Posted 25 October 2006 - 07:45 PM
You can use 1,2-Naphthoquinone-4-sulfonic acid sodium salt , aka 3,4-Dihydro-3,4-dioxo-1-naphthalenesulfonic acid sodium salt , known to its friends as Folin's Reagent or C10H5NaO5S for short.
Sigma-Aldrich stock it http://www.sigmaaldrich.com/catalog/search...382&Brand=FLUKA but do not quote a price online (bad sign ?
)
If it turns out to be another name for ninhydrin, I apologise but I think it is a genuine alternative.
Cheers
Sigma-Aldrich stock it http://www.sigmaaldrich.com/catalog/search...382&Brand=FLUKA but do not quote a price online (bad sign ?

If it turns out to be another name for ninhydrin, I apologise but I think it is a genuine alternative.
Cheers
#4
Posted 26 October 2006 - 07:23 PM
Thanks for the advice!! My teacher wasn't to keen on using it because it was carcinogenic. It has been used before in the school but always by teachers. I think he was a bit wary of a pupil using it.
#5
Posted 26 October 2006 - 07:26 PM
undermining or what!?
"It's Five O'Clock Somewhere..."
#6
Posted 13 November 2006 - 04:02 AM
if you want to develop amino acids without ninhydrin, try using iodine crystals, but do it in a closed jar. This might not be as good as ninhydrine, but it should be fine.
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